R1-002 (PD-L1)
Programmed cell death 1 ligand 1 (PD-L1; CD274; B7-H1) is type I transmembrane glycoprotein composed of IgC- and IgV-type extracellular domains and it is a member of the B7 gene family. (1) (2) PD-L1, together with PD-L2, is a ligand for programmed cell death protein 1 (PD-1) (3) additionally PD-L1 can bind to T-lymphocyte activation antigen CD80. (4) PD-L1 has very broad expression, it is constitutively expressed on murine and human antigen presenting cells, non-hematopoietic cells and non-lymphoid organs such as heart, lung, placenta and liver. (5) (4) It is often overexpressed on the surface of different tumor types including lymphoma, melanoma, lung and breast cancer, glioblastoma, ovarian, kidney tumors, and bladder cancers. (6) The expression is upregulated by type I and type II interferons (IFNs). (3) Formation of PD-1/PD-L1 complex leads to the inhibition of TCR-mediated lymphocyte proliferation and cytokine secretion. (2) PD-L1 plays a critical role in the induction and maintenance of peripheral transplant tolerance (5).
- Okazaki T and Honjo T. PD-1 and PD-1 ligands: from discovery to clinical application. International Immunology. 2007, Vol. 19, 7, pp. 813–824.
- Freeman GJ et al. Engagement of the PD-1 Immunoinhibitory Receptor by a Novel B7 Family Member Leads to Negative Regulation of Lymphocyte Activation. Journal of Experimental Medicine. 2000, Vol. 192, 7, pp. 1027-1034.
- Keir ME et al. PD-1 and Its Ligands in Tolerance and Immunity. The Annual Review of Immunology. 2008, 26, pp. 677–704.
- Ceeraz S et al. B7 family checkpoint regulators in immune regulation and disease. Trends in Immunology. 2013, Vol. 34, 11, pp. 556-563.
- Riella LV et al. Role of the PD-1 Pathway in the Immune Response. American Journal of Transplantation. 2012, 12, pp. 2575–2587.
- Zak KM et al. Structural Biology of the Immune CheckpointReceptor PD-1 and Its Ligands PD-L1/PD-L2. Structure. 2017, 25, pp. 1163-1174.
Sequence (Q9NZQ7)
MRIFA – secretion signal sequence
RLRKG – intracellular domain
FTVTV – ectodomain
C — C – disulfide bond
THLVI – transmembrane domain
N – glycosylation site
| Cat number | Protein construct | Tag | Expression Host | Order |
|---|---|---|---|---|
| R1-002-1 | PD-L1 ECD (19 – 238) | His-tag, Fc-tag | CHO K1 | Order |
| R1-002-1a | PD-L1 ECD (19 – 238) | His-tag | CHO K1 | Order |
| R1-002-2 | PD-L1 ECD (19 – 238) | Fc-tag | CHO K1 | Order |
| R1-002-2a | PD-L1 ECD (19 – 238) | Tag free | CHO K1 | Order |
| R1-002-3 | PD-L1 ECD (19 – 134) | His-tag | E. coli | Order |
| R1-002-3.1 (15N) | PD-L1 ECD (19 – 134) | His-tag | E. coli | Order |