Other recombinant proteins

/Other recombinant proteins
Other recombinant proteins 2019-02-26T13:44:05+00:00

Several recombinant proteins are available in Recepton for research and development applications:

  • Mdm2 (1-125)
  • Mdm2 (1-118)
  • Mdm2 (18-125)
  • Mdm4 (1-134)
  • CD44 (22-178)

E3 ubiquitin-protein ligase Mdm2

Product nameMdm-2 (human) (1-125)
UniProt IDQ00987
NatureRecombinant

Mdm2 (human) was expressed and purified from E. coli. The protein was expressed as inclusion bodies, which were purified and refolded. Final protein was purified by size exclusion chromatography.

Concentration0.1 mg/ml
MW14,1 kDa
Puritycrystallization grade
Storage bufferPhosphate buffer:
50 mM Na2HPO4
50 mM KH2PO4
150 mM NaCl
5 mM DTT
pH 7.4
Tris buffer:
5 mM Tris-HCl
50 mM NaCl
5 mM β-mercaptoethanol
pH 8.0
Stability and StorageStore at -80ºC. Snap refreeze in liquid nitrogen. Avoid multiple thaw or freeze cycles.

Literature

Twarda-Clapa A. et. al. (2017). 1,4,5-Trisubstituted Imidazole-Based p53-MDM2/MDMX,
Antagonists with Aliphatic Linkers for Conjugation with Biological Carriers. J Med Chem., 60(10), 4234-4244.

E3 ubiquitin-protein ligase Mdm2

Product nameMdm2 (human) (MAHHHHHHVDDDDKM-1-118)
UniProt IDQ00987
NatureRecombinant

Mdm-2 (human) was expressed and purified from E. coli. The protein was expressed as inclusion bodies, which were purified and refolded. Final protein was purified by size exclusion chromatography.

Concentration0.1 mg/ml
MW15,2 kDa
Purity≥ 95%
Storage bufferPhosphate buffer:
50 mM Na2HPO4
50 mM KH2PO4
150 mM NaCl
5 mM DTT
pH 7.4
Tris buffer:
5 mM Tris-HCl
50 mM NaCl
5 mM β-mercaptoethanol
pH 8.0
Stability and StorageStore at -80ºC. Snap refreeze in liquid nitrogen. Avoid multiple thaw or freeze cycles.

Literature

Twarda-Clapa A. et. al. (2017). 1,4,5-Trisubstituted Imidazole-Based p53-MDM2/MDMX, Antagonists with Aliphatic Linkers for Conjugation with Biological Carriers. J Med Chem., 60(10), 4234-4244.
Surmiak E. et. al. (2017). Rational design and synthesis of 1,5-disubstituted tetrazoles as potent inhibitors of the MDM2-p53 interaction. Eur J Med Chem, 126, 384-407.
Surmiak E. et al. (2016). A Unique Mdm2-Binding Mode of the 3-Pyrrolin-2-one- and 2-Furanone-Based Antagonists of the p53-Mdm2 Interaction. ACS Chem Biol, 11(12), 3310-3318.

E3 ubiquitin-protein ligase Mdm2

Product nameMdm2 (human) (18-125)
UniProt IDQ00987
NatureRecombinant

Mdm2 (human) was expressed and purified from E. coli. The protein was expressed as inclusion bodies, which were purified and refolded. Final protein was purified by size exclusion chromatography.

Concentration0.1 mg/ml
MW12,6 kDa
Puritycrystallization grade
Storage bufferPhosphate buffer:
50 mM Na2HPO4
50 mM KH2PO4
150 mM NaCl
5 mM DTT
pH 7.4
Tris buffer:
5 mM Tris-HCl
50 mM NaCl
5 mM β-mercaptoethanol
pH 8.0
Stability and StorageStore at -80ºC. Snap refreeze in liquid nitrogen. Avoid multiple thaw or freeze cycles.

Literature

Twarda-Clapa A. et. al. (2017). 1,4,5-Trisubstituted Imidazole-Based p53-MDM2/MDMX, Antagonists with Aliphatic Linkers for Conjugation with Biological Carriers. J Med Chem., 60(10), 4234-4244.

E3 ubiquitin-protein ligase Mdm4

Product nameMdm4 (human) (MAHHHHHHVDDDDKM-1-134)
UniProt IDO15151
NatureRecombinant

Mdm4 (human) was expressed and purified from E. coli. The protein was purified by immobilized metal-chelate chromatography (IMAC, Ni-NTA) under native conditions followed by size exclusion chromatography.

Concentration0.1 mg/ml
MW16,5 kDa
Puritycrystallization grade
Storage bufferPhosphate buffer:
50 mM Na2HPO4
50 mM KH2PO4
150 mM NaCl
5 mM DTT
pH 7.4
Tris buffer:
5 mM Tris-HCl
50 mM NaCl
5 mM β-mercaptoethanol
pH 8.0
Stability and StorageStore at -80ºC. Snap refreeze in liquid nitrogen. Avoid multiple thaw or freeze cycles.

Literature

Twarda-Clapa A. et. al. (2017). 1,4,5-Trisubstituted Imidazole-Based p53-MDM2/MDMX, Antagonists with Aliphatic Linkers for Conjugation with Biological Carriers. J Med Chem., 60(10), 4234-4244.

Cell surface receptor CD44

Product nameCD44 (human) (MGHHHHHHHHHHSSGHIDDDDKHM-22-178)
UniProt IDP16070
NatureRecombinant

CD44 (human) was expressed and purified from E. coli. The protein was expressed as inclusion bodies, which were purified and refolded. Final protein was purified by size exclusion chromatography.

Concentration0.1 mg/ml
MW20,3 kDa
Purity> 95 %
Storage bufferPhosphate buffer:
137 mM NaCl
2.7 mM KCl
10.0 mM Na2HPO4
1.8 mM KH2PO4
pH 7.4
Stability and StorageStore at -80ºC. Snap refreeze in liquid nitrogen. Avoid multiple thaw or freeze cycles.