Several recombinant proteins are available in Recepton for research and development applications:
- Mdm2 (1-125)
- Mdm2 (1-118)
- Mdm2 (18-125)
- Mdm4 (1-134)
- CD44 (22-178)
E3 ubiquitin-protein ligase Mdm2
| Product name | Mdm-2 (human) (1-125) |
| UniProt ID | Q00987 |
| Nature | Recombinant |
Mdm2 (human) was expressed and purified from E. coli. The protein was expressed as inclusion bodies, which were purified and refolded. Final protein was purified by size exclusion chromatography.
| Concentration | 0.1 mg/ml |
| MW | 14,1 kDa |
| Purity | crystallization grade |
| Storage buffer | Phosphate buffer: 50 mM Na2HPO4 50 mM KH2PO4 150 mM NaCl 5 mM DTT pH 7.4 Tris buffer: 5 mM Tris-HCl 50 mM NaCl 5 mM β-mercaptoethanol pH 8.0 |
| Stability and Storage | Store at -80ºC. Snap refreeze in liquid nitrogen. Avoid multiple thaw or freeze cycles. |
Literature
Twarda-Clapa A. et. al. (2017). 1,4,5-Trisubstituted Imidazole-Based p53-MDM2/MDMX,
Antagonists with Aliphatic Linkers for Conjugation with Biological Carriers. J Med Chem., 60(10), 4234-4244.
E3 ubiquitin-protein ligase Mdm2
| Product name | Mdm2 (human) (MAHHHHHHVDDDDKM-1-118) |
| UniProt ID | Q00987 |
| Nature | Recombinant |
Mdm-2 (human) was expressed and purified from E. coli. The protein was expressed as inclusion bodies, which were purified and refolded. Final protein was purified by size exclusion chromatography.
| Concentration | 0.1 mg/ml |
| MW | 15,2 kDa |
| Purity | ≥ 95% |
| Storage buffer | Phosphate buffer: 50 mM Na2HPO4 50 mM KH2PO4 150 mM NaCl 5 mM DTT pH 7.4 Tris buffer: 5 mM Tris-HCl 50 mM NaCl 5 mM β-mercaptoethanol pH 8.0 |
| Stability and Storage | Store at -80ºC. Snap refreeze in liquid nitrogen. Avoid multiple thaw or freeze cycles. |
Literature
Twarda-Clapa A. et. al. (2017). 1,4,5-Trisubstituted Imidazole-Based p53-MDM2/MDMX, Antagonists with Aliphatic Linkers for Conjugation with Biological Carriers. J Med Chem., 60(10), 4234-4244.
Surmiak E. et. al. (2017). Rational design and synthesis of 1,5-disubstituted tetrazoles as potent inhibitors of the MDM2-p53 interaction. Eur J Med Chem, 126, 384-407.
Surmiak E. et al. (2016). A Unique Mdm2-Binding Mode of the 3-Pyrrolin-2-one- and 2-Furanone-Based Antagonists of the p53-Mdm2 Interaction. ACS Chem Biol, 11(12), 3310-3318.
E3 ubiquitin-protein ligase Mdm2
| Product name | Mdm2 (human) (18-125) |
| UniProt ID | Q00987 |
| Nature | Recombinant |
Mdm2 (human) was expressed and purified from E. coli. The protein was expressed as inclusion bodies, which were purified and refolded. Final protein was purified by size exclusion chromatography.
| Concentration | 0.1 mg/ml |
| MW | 12,6 kDa |
| Purity | crystallization grade |
| Storage buffer | Phosphate buffer: 50 mM Na2HPO4 50 mM KH2PO4 150 mM NaCl 5 mM DTT pH 7.4 Tris buffer: 5 mM Tris-HCl 50 mM NaCl 5 mM β-mercaptoethanol pH 8.0 |
| Stability and Storage | Store at -80ºC. Snap refreeze in liquid nitrogen. Avoid multiple thaw or freeze cycles. |
Literature
Twarda-Clapa A. et. al. (2017). 1,4,5-Trisubstituted Imidazole-Based p53-MDM2/MDMX, Antagonists with Aliphatic Linkers for Conjugation with Biological Carriers. J Med Chem., 60(10), 4234-4244.
E3 ubiquitin-protein ligase Mdm4
| Product name | Mdm4 (human) (MAHHHHHHVDDDDKM-1-134) |
| UniProt ID | O15151 |
| Nature | Recombinant |
Mdm4 (human) was expressed and purified from E. coli. The protein was purified by immobilized metal-chelate chromatography (IMAC, Ni-NTA) under native conditions followed by size exclusion chromatography.
| Concentration | 0.1 mg/ml |
| MW | 16,5 kDa |
| Purity | crystallization grade |
| Storage buffer | Phosphate buffer: 50 mM Na2HPO4 50 mM KH2PO4 150 mM NaCl 5 mM DTT pH 7.4 Tris buffer: 5 mM Tris-HCl 50 mM NaCl 5 mM β-mercaptoethanol pH 8.0 |
| Stability and Storage | Store at -80ºC. Snap refreeze in liquid nitrogen. Avoid multiple thaw or freeze cycles. |
Literature
Twarda-Clapa A. et. al. (2017). 1,4,5-Trisubstituted Imidazole-Based p53-MDM2/MDMX, Antagonists with Aliphatic Linkers for Conjugation with Biological Carriers. J Med Chem., 60(10), 4234-4244.
Cell surface receptor CD44
| Product name | CD44 (human) (MGHHHHHHHHHHSSGHIDDDDKHM-22-178) |
| UniProt ID | P16070 |
| Nature | Recombinant |
CD44 (human) was expressed and purified from E. coli. The protein was expressed as inclusion bodies, which were purified and refolded. Final protein was purified by size exclusion chromatography.
| Concentration | 0.1 mg/ml |
| MW | 20,3 kDa |
| Purity | > 95 % |
| Storage buffer | Phosphate buffer: 137 mM NaCl 2.7 mM KCl 10.0 mM Na2HPO4 1.8 mM KH2PO4 pH 7.4 |
| Stability and Storage | Store at -80ºC. Snap refreeze in liquid nitrogen. Avoid multiple thaw or freeze cycles. |